Discovering Rare Variants by Use of Melting Temperature Shifts Seen in Melting Curve Analysis
نویسندگان
چکیده
منابع مشابه
Discovering rare variants by use of melting temperature shifts seen in melting curve analysis.
Two reports in this issue of Clinical Chemistry describe novel mutations in thrombophilia genes (1, 2). The authors of the reports describe variants detected by the identification of a melting temperature (Tm) different from the expected Tm of the target mutation and from the Tm of the wild-type sequence. The variants reported in this issue are A20218G in the prothrombin (factor II) gene and C1...
متن کاملDetecting rare thrombophilia variants by high-resolution melting.
Genotyping by amplicon melting is a minimalistic approach to geno-typing, requiring only PCR, a fluorescent DNA dye, and single-color acquisition of a melting curve in a closed tube. Multiplex genotyping for the F5 1 [coagulation factor V (proaccelerin, labile factor)] Le-iden (1691GϾA), F2 [coagulation factor II (thrombin)] 20210GϾA, and MTHFR [methylenetetrahy-drofolate reductase (NAD(P)H)] 1...
متن کاملIn-tube DNA methylation profiling by fluorescence melting curve analysis.
BACKGROUND Most PCR assays for detection of 5-methylcytosine in genomic DNA entail a two-step procedure, comprising initial PCR amplification and subsequent product analysis in separate operations that usually require manual transfer. These methods generally provide information about methylation of only a few CpG dinucleotides within the target sequence. METHODS An in-tube methylation assay i...
متن کاملGenotyping of ovine prion protein gene (PRNP) variants by PCR with melting curve analysis.
BACKGROUND Scrapie is the transmissible spongiform encephalopathy in sheep. Because genetic variants of the ovine PrP gene (PRNP) can be associated with disease risk, the European Union initiated programs to eradicate high-risk PRNP genotypes from sheep livestock. For this purpose, reliable and cost-effective genotyping is needed. METHODS We amplified DNA to cover the 3 risk codons in exon 3 ...
متن کاملSurface Melting Curve Analysis with R
Nucleic acid Melting Curve Analysis is a powerful method to investigate the interaction of double stranded nucleic acids. Many researchers rely on closed source software which is not ubiquitously available, and gives only little control over the computation and data presentation. R in contrast, is open source, highly adaptable and provides numerous utilities for data import, sophisticated stati...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2005
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2005.051177